KMID : 1103720070560050505
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Journal of the Korean Society of Radiology 2007 Volume.56 No. 5 p.505 ~ p.513
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Development of Microbubble Contrast Agents for High Frequency Ultrasound Microscopy
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Jun Se-Jung
Kim Eun-Ah Park Seong-Hoon Lee Hye-Jin Jun Hong-Young Byun Seung-Jae Yoon Kwon-Ha
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Abstract
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Purpose: To develop optimal microbubble contrast agents (MBCAs) for performing ultrasound microscopy when examining small animals.
Materials and Methods: We prepared three types of MBCAs. First, a mixture of three parts of 40% dextran and one part of 5% human serum albumin were sonicated with perfluorocarbon (PFC) (MB1-D40A5P). Second, three parts of 40% dextran and one part of 1% human serum albumin were sonicated with PFC (MB2-D40A1P). Third, all parts of 1% bovine serum albumin were sonicated with PFC (MB3-A1P). We measured the microbubbles¡¯ sizes and concentrations with using image analysis software. The acoustic properties of the microbubbles were assessed both in vitro and in vivo.
Results:The majority of the MB1-D40A5Ps had a diameter of 2-5 um, the mean diameter of the MB2-D40A1Ps was 2.5 um, and the mean diameter of the MB3-A1Ps was less than 2.0 um. Among the microbubbles, the MB1-D40A5Ps and MB2-D40A1Ps showed increased echogenicity in the abdominal vessels, but the duration of their contrast effect was less than 30 sec. On the contrary, the MB3-A1Ps exhibited strong enhancement in the vessels and their duration was greater than 120 sec.
Conclusions:A microbubble contrast agent consisting of all parts of 1% serum albumin sonicated with PFC is an effective contrast agent for ultrasound microscopy
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KEYWORD
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Ultrasound(US), contrast media, Microbubbles, Animals, Microsphere
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